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The container was stored in a 60C oven (Model DK-63 Constant Temperature Oven, Scientific Products, McGaw Park, IL)

Protein expression For whole cell lysates, cultured cells were rinsed with ice-cold PBS and lysed in RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with PhosSTOP and cOmplete protease inhibitor cocktail

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