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Following the removal of the His-SUMO tag by ULP1, the eluted protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 GL column with an elution buffer containing 20 mM HEPES (pH 7.4) and 200 mM NaCl

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Neutrophil Extracellular Traps Initiate Gallstone Formation