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One of these regulating factors is insulin-like growth factor-1

Flip the vial upside down while keeping needle inserted

Centrilobular (Figures 5459) Sometimes referred to as periacinar necrosis, it consists of irreversible cell death of centrilobular hepatocytes and is often seen after anoxia, or exposure to tannic acid, chloroform, or other hepatotoxic agents (Gopinath, Prentice, and Lewis 1987)