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Unfortunately as we age our levels of glutathione naturally decline

The expression of several proteins related to cell proliferation or differentiation, such as DDX11, RP1 and IFITM2, were also altered by riboflavin deficiency, which may be related to the changes in the cell viability we found in this study

After 2 min, 0.1 ml samples were diluted to a final volume of 1 ml containing 1 mM GSH, 1 mM CDNB in 0.1 M potassium phosphate buffer, pH 6.5, according to the standard procedure of Habig et al