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cDNA synthesized with the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific) was subjected to quantitative real time PCR with iQ SYBR Green Supermix (Biorad)

[ref] The highest sources of L-carnitine in the diet are meat and dairy products, with very little found in plant sources

Whole blood was layered on to ficoll histopaque in a 1:1 ratio and centrifuged at 25C for 30 minutes at 1800 RPM with the slow brake to prevent disturbance of the gradient

Effects of dietary polyphenol supplementation on iron status and erythropoiesis: a systematic review and meta-analysis of randomized controlled trials