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Pei Y, Agner BR, Luo B, Dong X, Li D, Liu J, et al

KawamuraSMatsushitaYKurosakiSTangeMFujiwaraNHayataYet al

Wherever possible, calibrate with defined oxidant fluxes (enzymatic generators), internal standards, or ratiometric reporters and fully report matrix and environmental conditions (buffer composition, protein content, temperature, pH, oxygen tension, light exposure) plus acquisition timing/kinetics to separate bursts from steady states (Kalyanaraman et al