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Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition

This imbalance results in chronic inflammation and can lead to leaky gut syndrome (5)

2.2 Functional implications of specific gut microbiota in hypothyroidism As seen in the results mentioned above, Phascolarctobacterium is more abundant in patients with hypothyroidism (Liu et al., 2020)

A rabbit model demonstrates the influence of cartilage thickness on intra-articular drug delivery and retention within cartilage