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Similarly, E2f1 / mice demonstrated impaired glucose tolerance when challenged with an oral bolus of glucose compared to E2f1 +/+ mice (Figure 1F and 1G) associated with an alteration of insulin secretion (Figure 1H) Strikingly, although insulin levels were dampened in E2f1 / mice, glucose-induced plasma Glp-1 levels were not affected by the deletion of E2f1 in cells (Figure 1I)

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This means that you will start with a small dose and gradually increase