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The ITC analysis confirmed that H35A mutation induced complete loss of binding affinity and K36A mutants had approximately 6.4 folds reduced affinity ( Kd value of 236 M) for NADPH compared with that of wild-type Pa DHDPR ( Kd value of 37 M) (Fig

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Figure 3 TEM images (Figure 4) demonstrated that the community was dominated by microorganisms with rod-like morphologies, presenting an undulated outer cell wall and accompanied by unidentified extracellular organic material