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Hypoglycemia Care: Ensure enough nutritional support around use times Consider dose reduction if hypoglycemia occurs Use glucose tracking protocols Have glucose use available if needed Injection Site Reaction Care: Rotate injection sites systematically Use proper injection technique Consider lower level solutions Apply cold compress if major reaction occurs Fluid Retention Care: Track body weight and signs of edema Consider dose reduction if major retention occurs Ensure enough hydration Track electrolyte balance Research Ethics and Informed Consent All research involving IGF1 LR3 should be conducted with appropriate ethical oversight and informed consent procedures

Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C

ensure pH and preservation are suitable

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