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HEK-293 cells (ATCC, #CRL-1573) were cultured in DMEM medium (Gibco) supplemented with 10% FBS and 100 U/mL penicillin/streptomycin

Metformin is a biguanide drug that decreases blood glucose by reducing glucose production in the liver, decreasing intestinal absorption, and increasing insulin sensitivity of target tissues

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Semaglutide is a once-weekly injection, ideally on the same day each week for consistency