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This decline correlates with reduced wound healing capacity, decreased collagen synthesis, and visible skin aging

[DOI] [PMC free article] [PubMed] [Google Scholar] 183.Nelson K.J., Perkins A., Van Swearingen A.E.D., Hartman S., Brereton A.E., Parsonage D., Salsbury F.R., Jr., Karplus P.A., Poole L.B

Research has also shown that NO exerts cytotoxic effects on respiratory epithelial cells and disrupts oxygen uptake by type II pneumocytes (93)

After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter