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After discarding the medium, cells were fixed with 4% paraformaldehyde for 30 min, permeabilized with 0.5% Triton X-100 for 10 min, and then the reaction system was prepared as per the instructions

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IGF-1 has been reported to be useful in the screening of GHD in some studies 3,4,5,6,7 but not in others 1,8

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