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At first, rubber stoppers are fitted on the necks of vials and then aluminum crimp seals are oriented over stoppers and crimped around vial necks by use of the crimping tool

Follow the specific instructions provided, and consult your doctor or healthcare professional if you have questions about the technique

Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol

For analysis, blood samples were categorized into three groups: (a) patients with serum samples collected under baseline conditions ( n = 32), (b) patients with serum samples collected at post-seizure ( n = 30), and (c) serum samples from healthy control subjects ( n = 23)