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Proteoliposome counterflow assays Frozen proteoliposome and protein-free liposome aliquots (lipid concentration: 8 mg/ml) were thawed and diluted twofold in internal liposome buffer with final concentrations of 150mM KCl, 50mM HEPES (pH 7.4), 5 mM (Figure 3a and Extended Data Figure 4l) or 10 mM (Extended Data Figure 7F) unlabeled GSSG (Thermo J63715.06)

Efpeglenatide and clinical outcomes with and without concomitant sodium-glucose co-transporter-2 inhibition use in type 2 diabetes: exploratory analysis of the AMPLITUDE-O trial

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This MEK-induced activation increases the expression of several downstream targets, including ELK-1, followed by an increase in c-fos, a growth cone-associated protein-43 that promotes neuronal plasticity