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Under this hypoxic and high-fat dietary environment, adipose tissue exhibits significant immune cell infiltration (macrophages, T cells) accompanied by characteristic phenotypic shifts ( Local hypoxia during adipocyte hypertrophy also induces endoplasmic reticulum stress (ERS) ( 4.3.2 Cytokine-mediated ROS generation and OS Inflammatory cytokines promote ROS production through multiple pathways ( ROS activate the NLRP3 inflammasome (37)

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Glutathione peroxidase 8 (GPx8) is a resident endoplasmic reticulum (ER) protein that introduces disulfide bonds into nascent proteins via protein disulfide isomerase (PDI)