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According to yet another embodiment of the present invention, the peptide Ac-Nle-cyc/o(- Asp-His-D-Phe-Arg-Trp-Lys)-OH is used in the manufacture of a medicament for intranasal administration for use in the treatment of erectile dysfunction in a male, wherein the peptide Ac-Nle- cyc/o(-Asp-His-D-Phe-Arg-Trp-Lys)-OH increases cGMP levels to an amount sufficient to produce an erection sufficient for sexual intercourse when the peptide Ac-Nle-cyc/o(-Asp-His-D-Phe-Arg-Trp- Lys)-OH is administered at a sub-therapeutic dose and administered subsequent to a sub- therapeutic dose of a PDE-5 inhibitor