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Non-targeting siRNA was used as a negative control

Relative contraindications requiring caution include active or suspected cancer (GHK-Cu promotes angiogenesis with concern about theoretically feeding tumors, though conflicting data shows documented anti-cancer gene expression effects requiring medical supervision), severe organ dysfunction (kidney or liver failure may slow peptide clearance), hemochromatosis or copper overload conditions, autoimmune disorders, and patients on immunosuppressants or blood thinners

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The two are complementary and non-redundant: GHK-Cu for dermis and adnexae, BPC-157+TB-500 for deep structures (tendons, ligaments, muscles, gut)