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Trypsin/LysC digestion and streptavidin enrichment Samples were digested by adding 3 l of 100 mM CaCl 2 (final concentration: 1 mM) and 2 g of trypsin/LysC (20 g trypsin/LysC resuspended in 40 l trypsin/LysC buffer, then adding 4 l to each sample), followed by shaking at 37 C for 16 hours

A positive regulatory domain in CCAAT/enhancer binding protein (C/EBP) is required for the glucocorticoid-mediated displacement of histone deacetylase 1 (HDAC1) from the C/ebp promoter and maximum adipogenesis