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To date, regulatory authorities have not issued warnings regarding blood clots and tirzepatide

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However, since cell counting is only a rough proxy to assess cytotoxicity and a higher dose escalation is necessary for CC 50 determination, we further included careful assessment of growth kinetics by live cell imaging, measurement of mitochondrial metabolic activity by MTT assay as well as cellular ATP levels by CellTiter-Glo (CTG, see Material and Methods section for details)