The library pool was quantified by real-time PCR using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System and then sequenced on an Illumina NovaSeq6000 sequencing instrument with a paired-end 2 150 bp
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Turk BE, Huang LL, Piro ET, Cantley LC: Determination of protease cleavage site motifs using mixture- based oriented peptide libraries