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Absence of immunoreactive vasoactive intestinal polypeptide in tissue from the lungs of patients with asthma

Epigenetics refers to long-lasting changes in gene expression that are regulated by transcriptional, post-transcriptional, intra- or post-translational mechanisms (such as DNA methylation, histone modifications, and microRNA regulation) that do not involve DNA sequence alteration and are usually characterized by stability and longevity [84]

El Ayed, M

For analysis of PhoA accumulation, 1 mL of each aforementioned cultures were harvested and the pellet was resuspended in standard 1x SDS-sample buffer adjusted to 100 L for an OD 600 of 1.0