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GSSG GSH (Reduced): Active antioxidant form GSSG (Oxidized): Inactive, recycled back to GSH via glutathione reductase Synthesis Pathway & Essential Cofactors: The Body's Complex Production Line Glutathione is produced in a multi-step enzymatic process, starting with homocysteine, which is converted to cysteine

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There is no thiol group (SH) in the conjugate product (CDNB-SG) molecule, and the conjugate product cannot bind onto the surface of AuNPs through AuS bond