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But for the prompt and willing response of UK breeders of the English toy spaniel to the challenge of an American, Roswell Eldridge, in 1926 (the same year the Queen was born), there would be no CKCS breed

After cryopreservation, motility, viability, mitochondrial activity, plasma membrane integrity, acrosome integrity, concentration of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT), and ability of frozen-thawed sperm cells to support in vitro embryonic development were evaluated