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Pernicious anemia , where the body cant absorb B12, is a main reason

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Athletes and individuals who exercise daily may have low B12 levels, due to the increased metabolism

The ON-switch was performed with 405 nm light 650 W/cm 2 for 0.5 ms, the OFF confinement with 4 ms of 488 nm light at 650 W/cm 2 and finally the read out with 240 kW/cm 2 of 488 nm per 1 ms