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J., OLoughlin, P

Inhibition of 5-LOX in a Cell-Free Assay The enzyme activity of human recombinant 5-LOX was determined spectrophotometrically by monitoring the oxidation of H 2 DCFDA to the highly fluorescent 2,7-dichlorofluorescein during the catalytic reaction mediated by 5-LOX as reported ( Inhibition of LTA 4 H in a Cell-Free Assay Leukotriene A4 hydrolase activity was measured by monitoring the formation of LTB 4 with an enzyme-linked immunosorbent assay

8 Conclusion Smooth muscle plays a central role in regulating physiological processes across multiple organ systems, and its dysfunction contributes significantly to the pathogenesis of respiratory, gastrointestinal, reproductive, and cardiovascular disorders

Consequently, novel anticancer drugs may target these transporters