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Prior to analysis, the XFe96 instrument (Seahorse Biosciences, North Billerica, MA, USA) mixed the assay media in each well for 10 minutes to allow the oxygen partial pressure to reach equilibrium

Researchers should protect the compound from excessive heat, moisture, and direct light exposure while following appropriate laboratory procedures

Contact your GP if you experience: Persistent fatigue, weakness, or shortness of breath (possible anaemia) Numbness, tingling, or balance problems (possible B12 deficiency) Muscle cramps, spasms, or irregular heartbeat (possible magnesium or potassium deficiency) Bone pain or frequent fractures (possible vitamin D or calcium deficiency) Poor wound healing or frequent infections (possible zinc or vitamin C deficiency) Seek urgent medical attention (call 111 or 999 as appropriate) for severe palpitations, chest pain, fainting, or signs of severe allergic reaction

This process is regulated by iron homeostasis, redox balance, lipid metabolism, glutathione metabolism, and various disease signaling pathways