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(10)Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (10) Preparation of Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (9)Fmoc-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Gly-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin in step (9), add a decapping reagent and react at 10-50C for 5-30 minutes , dry it with a vacuum pump, re-add the decapping reagent at 10-50 C for 10-60 minutes, drain it, wash it with isopropanol for 3 times, wash it with DMF for 3 times, wash it twice with DMF, add the solution dissolved in DMF Mixture of Fmoc-Gly-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt, react at 10-50C for 10-60 minutes, dry it with a vacuum pump, wash with isopropanol for 3 times, DMF for 3 times, The DMF that redistilled is washed 2 times, sucks dry, obtains Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin resin

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The question of which B12 form is 'best' depends largely on individual circumstances, the route of administration, and the specific clinical context

This calculator estimates concentration, dose volume in mL, syringe measurements, and estimated doses per vial