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Measurement of reactive oxygen species and lipid peroxidation Cells were incubated with 5 M BODIPY 581/591 C11 (Life technologies) for lipid peroxidation, or 5 M CM-H2DCFDA (Life technologies) for ROS in the dark for 30 minutes at 37 C

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A rational protocol is kisspeptin daily for ongoing hormonal optimization, with PT-141 as-needed for acute libido enhancement

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doi: 10.1111/jcpe.13864 112 LuoTGuoZLiuDGuoZWuQLiQet al

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