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You can use between 1mL and 3mL depending on your preference, but 2mL offers the best balance of convenience and accuracy

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This process and its linkage with cytosolic GSL synthesis requires two transport steps across the chloroplast envelope, as the first reaction allowing Met to enter biosynthesis of GSLs is a deamination of Met by a cytosolic BCAT4 (Schuster et al., 2006)