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this discrepancy is reasonable considering the yield of HGF after a wash-out step that often causes trapping/stacking of proteins on ultra-filtration membranes

Abbreviations Ahp alkyl hydroperoxide reductase APS 5-adenylylsulfate BSO buthionine sulfoximine CoA coenzyme A Cys Cysteine EGFR epidermal growth factor receptor ER endoplasmic reticulum FTR ferredoxin-thioredoxin reductase Gpx glutathione peroxidase Grx Glutaredoxin GSH reduced glutathione Gsr glutathione reductase GSSG oxidized glutathione, glutathione-disulfide Hcy Homocysteine LA lipoic Acid Lpd lipoic acid dehydrogenase Met Methionine Msr methionine-sulfoxide reductase NAD + nicotinamide adenine dinucleotide, oxidized NADH nicotinamide adenine dinucleotide, reduced NADP + nicotinamide adenine dinucleotide phosphate, oxidized NADPH nicotinamide adenine dinucleotide phosphate, reduced NOX NADPH-oxidase Nrf2 nuclear factor erythroid-2-related-2 PAPS 3-phosphoadenosine-5-phosphosulfate PDGFR platelet-derived growth factor receptor PPG Propargylglycine Prx Peroxiredoxin RNR ribonucleotide reductase ROS reactive oxygen species S Sulfur SAH S -adenosyl homocysteine SAM S -adenosyl methionine Se Selenium Sec Selenocysteine TCA tricarboxylic acid cycle Tgr thioredoxin-glutathione reductase TRP14 thioredoxin-related protein of 14 kDa Trx Thioredoxin TrxR thioredoxin reductase Footnotes Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication

W., Kim, Y

Free radicals are unstable These free radicals are chemically unstable, and highly reactive