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3, 61-90 degrees

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Centrifuge the cells at 80 g for 3 min and wash the pellet three times in Solution D (see Note 6)

It has also shown to exert protective effects on different experimental pathological situations, in which free radicals and ROS are involved, such as hyperacidity, parasites, constipation, musculoskeletal pain, arthritis, allergies, Ehlers-Dantos syndrome and immunomodulation [16, 17, 24]