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2) largely overlapped for intermediate until high concentrations of imidacloprid, i.e., 0.1 mg kg 1 dry soil and above, indicating similar effects of imidacloprid on springtail fecundity independent of DEM exposure

The membrane was treated with ECL prime blocking solutions (GE Healthcare, Little Chalfont, UK) and reacted overnight at 4 C with each antibody (Table S1) diluted in blocking buffer

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The peptide downregulates TNF- (6.2-fold reduction) and IL-6 (4.1-fold reduction) while upregulating anti-inflammatory IL-10 (8.1-fold increase)[12]