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Fusion primers with dual-indexed PCR adapters were used to generate the construct library, and short fragments were removed with AMPure beads

Additionally, injecting lentivirus expressing shRNA for lncRNA-H19 intra-articularly at the ankle of CIA mice ameliorates the progression of CIA by competitively binding with miR-124a, which directly targets CDK2 and MCP-1 (267)

Storage and Handling Requirements To safeguard the delicate peptide bonds and prevent structural degradation of the 10mg lyophilized cake, all vials must be stored long-term in a freezer environment at -20C

One of the peptides that triggers mast cells is leptin, which is produced at higher levels in people with more fat mass