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Implications of microglia in amyotrophic lateral sclerosis and frontotemporal dementia

For lysates preparation ( 31 ), cultures were centrifuged (10,000 g, 15 minutes), washed twice with potassium phosphate buffer (100 mM, pH 7.0), resuspended in borate buffer (50 mM, pH=8.4), and broken by mechanical lysis with glass beads in a tissue lyser (Qiagen, Courtaboeuf, France) 10 minutes, 50 Hz

Role for inducible nitric oxide synthase in protection from chronic chlamydia trachomatis urogenital disease in mice and its regulation by oxygen free radicals

Binding of Cu(II) ions to alpha-2 macroglobulin Our results demonstrated that 2M does not bind Cu(II) ions