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Following the initial "loading phase," our injections will cost approximately 20 per month (based on the 10 x 1mg kit with an injection once every 4 weeks)

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For immunofluorescent staining, tissue sections were incubated with anti-insulin antibody (Abcam), followed by a secondary fluorophore-conjugated antibodies (Invitrogen), or with FITC conjugated anti-mouse CD11c (BD Bioscience), or with Cy5 conjugated anti-mouse F4/80 (eBioscience) and counterstained with 4,6-diamidino-2-phenylindole (DAPI) (Sigma)

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