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Myxedema can be seen in 0.5-4.3% of patients with GD, and 13% of patients with TED (309, 310)

DT40 cellsfrom KJ Patels laboratory stockwere grown in RPMI medium supplemented with 3 % chicken serum (Gibco, # 16010167), 7 % Fetal calf serum (Gibco, # 16110082), 50 mol L 1 -mercaptoethanol, and P/S 11

Vitamin B12 is an essential nutrient that humans need to ingest on a regular basis because it is a water soluble vitamin that cannot be stored in your body