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Macaques that were euthanized at 3 days and 21 days had multifocal lung lesions, with alveolar septal thickening due to oedema and fibrin, small to moderate numbers of macrophages, a few neutrophils, minimal type II pneumocyte hyperplasia and some perivascular lymphocyte cuffing

PKA activity measurements PKA activity was measured using AKAR3 as previously described [22]

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Given that PI3K-AKT signaling regulates key downstream targets involved in cytokine production, cell survival, fibroblast-to-myofibroblast transition, and tissue remodeling in chronic airway diseases [39, 40], the observed inhibition of pathway phosphorylation provides a plausible explanation for the reduced airway inflammation and preservation of alveolar architecture in our model