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No saturation behavior for GSSG was previously detected for the fusion construct between rxYFP and ScGrx1 31 , which makes sense considering that PfGrx and many other glutaredoxins are at least two orders of magnitude more active than ScGrx1 (Table 1) 14,56

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Acknowledgements The authors' work is supported by the Primordia Institute of New Sciences and Medicine, by grants MOST103-2320-B-182-027-MY3 and MOST103-2321-B-182-014-MY3 from the Ministry of Science and Technology of Taiwan, and grants CMRPD1B0053, CMRPD1C0782, CMRPD190303, BMRPA04 and QZRPD120 from Chang Gung Memorial Hospital, Taiwan