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Specifically, BDNF was necessary for differentiation but not the proliferation of newborn neurons, as the only difference between BDNF knockdown and controls was the number of cells expressing both proliferation marker (BrdU) and immature neuronal marker (DCX), whereas the total number of proliferating cells, either 24 h (Figure 5b) or 7 days (Figure 5c) after BrdU injections, was unchanged

either a general-purpose medium such as Hanks Balanced Salt Solution (HBSS), which may be supplemented with Ca2+ if required, or a customized medium: Geys Balanced Salt Solution (GBSS) is routinely used for hepatic cells

Oncologist 2013

224 Mechanistically, mGluRs can directly regulate glutamatergic signalling by anchoring in complexes with SHANK and HOMER proteins and further control the synthesis of synaptic proteins via activation of the PI3K/AKT/mTOR pathways