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Absorption issues: People with mucous membrane lining disorders like Crohn's disease or gluten intolerance (celiac disease) or who have undergone gastrointestinal surgery are more likely to need vitamin B12 supplements as their absorption is impaired

Benussi A, Koch G, Cotelli M, Padovani A, Borroni B

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The 28-day multi-use window typically cited for Bacteriostatic Water-reconstituted compounds and reflecting the bacteriostatic preservation duration under recommended storage and handling conditions , covers the majority of standard pre-clinical research protocol durations