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In vitro covalent neddylation assay was performed as described in the Methods section to determine the effects of Smurf1, Smurf1-C426A and Smurf1C699A on PDK1 neddylation in cell-free system

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Demonstration of location of the MRS voxel in the parieto/occipital grey matter (A, B) and parietal white matter (B, C) on these coronal T2 (A, C) and sagittal three dimensional fast spoiled gradient echo (B, D) images of patient 1