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These limitations include cytotoxicity, immune rejection, regulatory obstacles, senescence and inflammation, and limited targeting precision [10, 58, 59]

In conclusion, the use of melatonin as an adjunctive therapy for sepsis treatment significantly reduced inflammatory biomarkers and improved clinical conditions in neonates, but larger scale studies with higher validity are needed to demonstrate clear clinical benefits of the therapy 103,105,107,108,109

The particles were recovered by centrifugation at 14,000 g for 15 min room temperature following freeze drying (Buchi, Lyovapor L-200)

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