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Stereotaxic surgery in mice After 810 weeks of the body weight monitoring protocol, Vgat-IRES-cre mice were individually anesthetized with an intraperitoneal injection of ketamine/xylazine (100/8 mg/kg), and the mice were then placed in a stereotaxic apparatus

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At the 2.5 mg starting dose, that concentration works perfectly because you only need 25 units

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