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For immunofluorescence staining, 2 different sections per mouse spaced by at least 120 m were co-stained with rabbit anti-insulin (1:500, Thermofisher, bs-0056R) and mouse anti-glucagon (1:500, Abcam, ab10922) primary antibodies overnight at 4C followed with a 1-hour incubation with the secondary antibodies Alexa Fluor 555 donkey anti-rabbit (1:400, Invitrogen, A21428) and Alexa Fluor 488 goat anti-mouse IgG1 (1:500, Invitrogen, 121121) at room temperature

Free radicals are unstable molecules that can cause cellular wear and tear over time

However, we do know that ingredients like green tea can cause nausea, abdominal distress, and even vomiting for people who arent used to the effects of caffeine

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