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coli cells were harvested by centrifugation at 5,200 g for 15 min and resuspended in 30 ml of A10 buffer (50 mM sodium phosphate buffer, 150 mM NaCl and 10 mM imidazole, pH 8.0) containing one tablet of Pierce protease inhibitor (Thermo Fisher Scientific)

[8] : 155165 Vitamin C was discovered in 1912, isolated in 1928, and in 1933, was the first vitamin to be chemically produced

[DOI] [PubMed] [Google Scholar] 70.Meyer F., Galan P., Douville P., Bairati I., Kegle P., Bertrais S., Czernichow S., Hercberg S

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