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Converting a nanomoles-per-kilogram protocol dose to milligrams requires multiplying the desired nmol/kg figure by the subject's weight in kilograms, then multiplying by the molecular weight (849 g/mol), and dividing by 1,000,000 to arrive at milligrams

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Purification and Detection High performance liquid chromatography (HPLC) purification: The synthesized polypeptides need to be purified by HPLC to remove impurities and unreacted raw materials, ensuring that the purity meets the pharmaceutic-grade standard