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In animal-model work, NNMT inhibition with 5-Amino-1MQ has been associated with changes in adipose-tissue mass, in NAD+ pool sizes (because NNMT consumes nicotinamide that would otherwise feed the NAD+ salvage pathway), and in methyl-donor dynamics (because NNMT consumes S-adenosylmethionine)

Across approximately 900 participants, the adverse event profile was indistinguishable from placebo

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It found that B12 deficiency was higher in vegans than vegetarians and that vegetarians report B12 deficiency irrespective of their demography, where they live, their age, and the type of vegetarian diet they eat