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Designing GLP-1 delivery: structural perspectives and formulation approaches for optimized therapy

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At 48-hour post-treatment, the cell culture supernatant was collected and tested on Human IFN gamma ELISA Kit (Invitrogen), Human IL-2 ELISA Kit (Acro Biosystems), and Human TNF alpha Uncoated ELISA Kit (Invitrogen) to measure IFN-, IL-2 and TNF-, respectively, according to the manufacturer instruction