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Imaging and figure preparation Images of hypothalamic cultures were collected on the calcium imaging microscope as described above, and separate cultures were plated onto PhenoPlateTM 96-well microplates (Perkin Elmer), fixed in 4% paraformaldehyde (Thermo Fisher Scientific) for 10 minutes, rinsed three times with Tris-buffered saline containing 0.1% Triton X-100 (TBS-T

Bioavailability is the most critical factor

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Elevating LPCAT3 mRNA levels can mitigate chondrocyte ferroptosis in OA, and its enzymatic activity correlates with local inflammatory levels